g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram A Possible Antidote for Tylenol (Paracetamol) Overdose American Council on Science and Health Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen and aspirin toxicity are both high yield, but the injury pattern is very different. Acetaminophen overdose overwhelms glutathione, allowing NAPQI to build up and cause hepatic necrosis. The antidote is N acetylcysteine, which Researchers are studying a possible link between acetaminophen (Tylenol) use during pregnancy and higher autism or ADHD risk. It may lower glutathione, an important antioxidant for brain development.