Metabolism: Master the Carnitine Shuttle and Stop Exam Stress! Long Chain Fatty Acids (LCFAs) love to burn energy, but they face a major obstacle: they cannot enter the mitochondrial matrix where Beta Oxidation Short Note On Carnitine Shuttle: Fatty acids are oxidized in mitochondria, and synthesized in the cytoplasm. Long chain fatty acids must form an active intermediate, fatty acyl CoA, before being oxidized inside mitochondria. Catherine Carnitine, Acylcarnitines and Beta Oxidation Carnitine Shuttle an overview ScienceDirect Topics A Deficiency of CarnitineAcylcarnitine Translocase in the Inner Mitochondrial Membrane New England Journal of Medicine