Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Sex differences in glutathione metabolism and acetaminophen toxicity Acetaminophen, Acetylcysteine, and Anaphylaxis With a Twist BROWN EMERGENCY MEDICINE BLOG Acetaminophen To Use or Not to Use? SEE Alternatives, LLC